SubtiBank SubtiBank
floT [2017-12-02 18:33:39]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

floT [2017-12-02 18:33:39]

membrane-associated scaffold protein, orchestration of physiological processes in lipid microdomains, involved in the control of membrane fluidity, confers (together with YuaF) resistance to cefuroxime
locus
BSU31010
pI
5.14
mw
55.82 kDa
protein length
509 aa Sequence Blast
gene length
1527 bp Sequence Blast
function
involved in the control of membrane fluidity
product
membrane-associated scaffold protein
essential
no
synonyms
yuaG, yuaH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.7|Membrane dynamics]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.5|Other proteins required for biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,180,465 → 3,181,994

    Phenotypes of a mutant

  • delayed onset of sporulation, reduced sporulation frequency
  • defect in motility [Pubmed|22753055]
  • reduced [SW|protein secretion] [Pubmed|23651456]
  • a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant does not induce [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|KinC]-dependent biofilm formation upon addition of surfactin [Pubmed|20713508]
  • a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency [Pubmed|22753055]
  • a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant has a [SW|sporulation] defect, due to the lack of [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|FtsH] [Pubmed|22882210]
  • a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]'' mutant displays a defective growth under oxygen-limiting conditions [Pubmed|25909364]

    • The protein

    Catalyzed reaction/ biological activity

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]-dependent expression of ''[gene|5D5D9A544295DD9B55C75A8CF47AB19935E740C6|fabF]'' and the ''[gene|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]-[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]-[gene|56345EA776E1C5664EF1F2D1AF5CE3E5F40AD442|yuaI]'' operon result in reduced membrane fluidity [Pubmed|21542858,22178969]
  • recruits [protein|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|YuaF] to focal assemblies [Pubmed|22753055]
  • controls protease activity of [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|FtsH] [Pubmed|24222488]

    • [SW|Localization]

  • membrane associated [Pubmed|18763711]
  • co-localizes with [protein|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|FloA] and [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|KinC] in discrete foci membrane [Pubmed|20713508]
  • forms discrete focal structures in the cytoplasma membrane [Pubmed|22753055]
  • 6 foci [protein|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|FloT] of are detected in strain 3610 [Pubmed|25909364]

    • Expression and Regulation

    Operons

    genes
    [gene|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]-[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]-[gene|56345EA776E1C5664EF1F2D1AF5CE3E5F40AD442|yuaI]
    description
    [Pubmed|9987136]

    sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|9987136], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

    • regulation

  • expressed upon cell wall stress ([protein|search|SigW]) [Pubmed|9987136]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A213 (yuaG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/213 NBRP B. subtilis, Japan]
  • JS152 (markerless), available in [SW|Daniel Lopez]'s lab
  • BKE31010 (Δ[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAAATTCCTCCTTTT, downstream forward: _UP4_GAGTAAGGAAAGGGCAGAAC
  • BKK31010 (Δ[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAAATTCCTCCTTTT, downstream forward: _UP4_GAGTAAGGAAAGGGCAGAAC

    • GFP fusion

  • GK38 (168 ''amyE''::P''floT-yfp'' (spc)), available in [SW|Daniel Lopez]'s lab
  • JS280 (3610 ''amyE::floT-gfp''(spc)), available in [SW|Daniel Lopez]'s lab
  • JS153 (3610 ''lacA::floT''-mEos2 (mls)), available in [SW|Daniel Lopez]'s lab
  • JS166 (3610 ''lacA::floT''-PAmCherry (mls)), available in [SW|Daniel Lopez]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]) for FloT [Pubmed|25909364], available in [SW|Daniel Lopez]'s lab

    • References

    Reviews

  • 25652542

    • Original publications

  • 9987136,22753055,12107147,18763711,19383680,20713508,23651456,22178969,23249255,22882210,25635948,25909364,24222488,26297017,27362352